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/2 mice in the expected Mendelian ratio, at the least at time of weaning. When mice in this colony have been examined for fertility, we found that each male and female Anp32e2/2 mice of 640 weeks of age were in a position to make normal-sized litters at typical frequencies when bred to their Anp32+/2 counterparts. These findings suggest that loss of Anp32e function will not deleteriously affect th
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s had been washed with cold PBS and lysed with Laemmli buffer. Lysates had been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin have been utilised as principal antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies have been used as secondary antibodies. Immunoreactive bands had been revealed working with the SuperSignalH
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s have been washed with cold PBS and lysed with Laemmli buffer. Lysates have been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin have been utilized as major antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been used as secondary antibodies. Immunoreactive bands have been revealed working with the SuperSignalH We
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s were washed with cold PBS and lysed with Laemmli buffer. Lysates had been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin had been utilised as major antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been utilized as secondary antibodies. Immunoreactive bands were revealed utilizing the SuperSignalH West Femtoche
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s were washed with cold PBS and lysed with Laemmli buffer. Lysates had been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin have been utilized as principal antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been employed as secondary antibodies. Immunoreactive bands have been revealed using the SuperSignalH West Fe