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/2 mice in the expected Mendelian ratio, at the least at time of weaning. When mice in this colony have been examined for fertility, we found that each male and female Anp32e2/2 mice of 640 weeks of age were in a position to make normal-sized litters at typical frequencies when bred to their Anp32+/2 counterparts. These findings suggest that loss of Anp32e function will not deleteriously affect th
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s have been washed with cold PBS and lysed with Laemmli buffer. Lysates have been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin have been utilized as major antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been used as secondary antibodies. Immunoreactive bands have been revealed working with the SuperSignalH We
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s were washed with cold PBS and lysed with Laemmli buffer. Lysates had been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin had been utilised as major antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been utilized as secondary antibodies. Immunoreactive bands were revealed utilizing the SuperSignalH West Femtoche
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