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s have been washed with cold PBS and lysed with Laemmli buffer. Lysates have been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin have been utilized as major antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been used as secondary antibodies. Immunoreactive bands have been revealed working with the SuperSignalH We
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s were washed with cold PBS and lysed with Laemmli buffer. Lysates had been then resolved by 15% SDS-PAGE and transferred to a nitrocellulose membrane. Cleaved caspase-3 and a-tubulin had been utilised as major antibodies. Horseradish-peroxidase-labeled rabbit and mouse antibodies had been utilized as secondary antibodies. Immunoreactive bands were revealed utilizing the SuperSignalH West Femtoche
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